电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进肿瘤细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简述 ：探讨强磁场是否可通过转化酪氨酸-β（TGF-β）-内皮细胞的内膜-泌尿系统转换 (EMT)来促使恶性肿瘤的波及迁移。使用总量2Gy(60)Coγ线紫外线照射源自人类所循环系统的6种恶性肿瘤，记录与EMT相关的变化，这除此以外分别透过光学新技术，细胞质印迹方法，免疫荧光新技术，划痕试验中和Transwell鲍洛通试验中来观察并检测细胞组织形态，EMT标上，波及迁移意志力等。采用酶亦同免疫吸附法检测这些恶性肿瘤之中TGF-β细胞准确度，透过特别减缓剂SB431542来评估TGF-β信号通道在强磁场EMT之中的作用。经过总量为2Gy紫外线照射的恶性肿瘤之中存在间叶细胞的表达，与假紫外线照射组相比其内膜标上减少，间叶细胞标上增加，同时其波及转移意志力提升，TGF-β细胞准确度也提高。进一步断定由A549强磁场可借的EMT可通过对TGF-β信号减缓发生逆转。这些试验中中TGF-β内皮细胞的EMT在强磁场可借提升恶性肿瘤波及转移意志力之中起着关键人物。

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